MiSeq

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The Illumina MiSeq

A standard Illumina MiSeq run produces paired-end data with read lengths up to 150bp. The run time for a standard 2x150bp run is 24 hours. Currently there is normally no queue to use this instrument, as it only requires one sample to run.

Sanger-encoded fastq.gz files are made available for user collection, with aligned data available on request. A typical MiSeq run will produce 3.5 Gbp of raw sequence data (approximately 23 million reads).

Hiseq vs MiSeq data comparison

Machine Data generation
HiSeq 3000 Typically sequences more than 350 million inserts per lane from a 2x150 bp sequencing run.
HiSeq 2500 Typically sequences more than 250 million inserts per lane from a 2x100 bp sequencing run.
NextSeq Typically sequences more than 400 million inserts per lane from a 1x75 bp sequencing run.
MiSeq Typically sequences more than 20 million inserts per lane from a 2x150 bp sequencing run .