Library preparation

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The library preparation laboratory

We are currently experienced in a wide range of library production techniques and aim to add more methodologies to our repertoire as time progresses, allowing you access to the most up to date methods in your field. If you have any questions or suggestions please contact us.

We are able to offer a range of standard library preparation, but are also willing to help you create your own. Whether you require help troubleshooting, tips on best practice, or just an aliquot of a library preparation kit, we are prepared to help.

Below is a list of commonly used library preparation kits with possible suppliers if you wish to buy your own.

Overview of RNA based library production

A very brief summary of RNA based library production and rough suggestions on the HiSeq sequencing run parameters can be found here.

 

Please Note: All these kits contain the basic reagents required for library preparation, but ALL require further components to be purchased separately.

We recommend purchasing NEB kits from Science Warehouse and Illumina Kits directly from Illumina (by creating an online account at www.illumina.com).

If you would prefer to have your libraries made in house for you, there is a standard charge of £200 per sample for an exome library and £100 for other types of library plus the cost of reagents.

For any of the library preparation methods detailed above you must be aware of the type and number of samples you would like to sequence as well as to what depth of coverage. This information can help to determine which instrument you use for sequencing. We have extensive experience using these library preparation methods and are very happy to discuss your requirements and answers questions and queries (emails here).