Important note

The HiSeq 2500 will be decommissioned at the end of October with the libraries previously sequenced on the HiSeq 2500 analysed on our new NextSeq 500.



The Illumina HiSeq

We have both HiSeq 2500 and HiSeq 3000 sequences with our standard Illumina HiSeq 2500 run produces paired-end data with read lengths up to 100 bp, while the HiSeq 3000 typically generates 150 bp paired end sequence data. As soon as your Illumina-compatible sequencing libraries are prepared, please contact us to reserve your place in our sequencing queue. Samples are run on a first-come, first-served basis. The run time for a standard 2x100-bp run in rapid mode about 1 day, while the HiSeq 3000 runs take aprroximatly 3 days.

Sanger-encoded fastq.gz files are made available for user collection, with aligned data available on request. This includes a run information file that should be retained for future analysis.

Hiseq vs MiSeq data comparison

Machine Data generation
HiSeq 3000 Typically sequences more than 350 million inserts per lane from a 2x150 bp sequencing run.
HiSeq 2500 Typically sequences more than 250 million inserts per lane from a 2x100 bp sequencing run.
NextSeq Typically sequences more than 400 million inserts per lane from a 1x75 bp sequencing run.
MiSeq Typically sequences more than 20 million inserts per lane from a 2x150 bp sequencing run .