Important note

The HiSeq 2500 will be decommissioned at the end of October with the libraries previously sequenced on the HiSeq 2500 analysed on our new NextSeq 500.

HiSeq

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The Illumina HiSeq

We have both HiSeq 2500 and HiSeq 3000 sequences with our standard Illumina HiSeq 2500 run produces paired-end data with read lengths up to 100 bp, while the HiSeq 3000 typically generates 150 bp paired end sequence data. As soon as your Illumina-compatible sequencing libraries are prepared, please contact us to reserve your place in our sequencing queue. Samples are run on a first-come, first-served basis. The run time for a standard 2x100-bp run in rapid mode about 1 day, while the HiSeq 3000 runs take aprroximatly 3 days.

Sanger-encoded fastq.gz files are made available for user collection, with aligned data available on request. This includes a run information file that should be retained for future analysis.

Hiseq vs MiSeq data comparison

Machine Data generation
HiSeq 3000 Typically ~50 Gbp of raw sequence per lane from a 2x150-bp sequencing run (~350 million reads).
HiSeq 2500 Typically ~35 Gbp of raw sequence per lane from a 2x100-bp sequencing run (~250 million reads).
MiSeq Typically ~3.5 Gbp of raw sequence per lane from a 2x150-bp sequencing run (23 million reads).